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Figure 6 | BMC Microbiology

Figure 6

From: The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP

Figure 6

The active site of VCA0965 tolerates additional amino acid substitutions. (A) Substitution mutants of every amino acid in the first position of the AGDEF active site of VCA0965 (residue 284) were constructed and examined for the ability to reduce motility of V. cholerae. (B) The intracellular concentration of c-di-GMP from V. cholerae expressing WT VCA0965 or the GGDEF, HGDEF, and MGDEF mutants was determined using LC-MS/MS. Open bars are the uninduced conditions while gray bars are induced with 0.1 mM IPTG. Error bars indicate the standard deviation and statistical significance from the corresponding uninduced culture was determined using a one-tailed Student’s t-test (n = 3, *p < 0.05).

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