Figure 5From: The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP VCA0965 (180–396) is an active DGC in vitro . The cytoplasmic portion of VCA0965, VCA0965(180–396), was purified. This protein and WspR(R242A) were incubated with and without 12.5 µM GTP overnight. The concentration of synthesized c-di-GMP was determined by LC-MS/MS. The mean value and standard deviation are shown (n = 3).Back to article page