Figure 4From: The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP VCA0965 increases the intracellular concentration of c-di-GMP. Wild type VCA0965 and its mutant derivatives were overexpressed in V. cholerae grown to an OD600 of 0.6-1.0, and the resultant c-di-GMP levels were measured using LC-MS/MS. The mean and standard deviation are indicated (*= p < 0.05, n = 3).Back to article page