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Figure 3 | BMC Microbiology

Figure 3

From: The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP

Figure 3

Overexpression of the VCA0965-I mutant induces biofilm formation in an A-site dependent manner. (A) Mean biofilm formation of V. cholerae overexpressing wild type VCA0965 and its mutant derivatives were measured using a MBEC assay. (B) Biofilm formation as measured by flow cytometry was determined by multiplying the mean forward scatter value of the aggregate population by the percentage of aggregates relative to the total events measured. The mean and standard deviation are indicated (* = p < 0.05, n = 3).

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