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Figure 2 | BMC Microbiology

Figure 2

From: The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP

Figure 2

VCA0965 requires an intact active site to repress motility. Quantification of motility as determined by quantifying colony area (cm2) in V. cholerae strains expressing the indicated proteins from a Ptac promoter on a plasmid. Proteins were induced with 0.1 mM IPTG (gray bars). The mean and standard deviation are indicated (* = p < 0.05, n = 3). In this and all subsequent figures the strain designations are pCMW75 (QrgB) (an active DGC from V. harveyi), pVCA0965 (WT VCA0965 expression plasmid), pVCA0965-I (I-site mutant), pVCA0965-A (A-site mutant), and pVCA0965-AI (I-site/A-site double mutant).

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