PCR primer sequence (5′ → 3′) | |||||
---|---|---|---|---|---|
Geneª | SNP locationª | PCRb | Amplicon (bp)b | Forwardb | Reverseb |
dnaA:dnaN | 1977 | Multiplex 1 | 131 | [M13] - TGAGAAGCTCTACGGTTGTTGTTCG | TTTCACCTCACGATGAGTTCGATCC |
(Rv0001:Rv0002) | |||||
Rv0260c | 311613 | 114 | CACCACTGTTGCCACGATGTTCTT | [M13] - GGCGACTTGCTACGCGTCCTAC | |
icd2 (Rv0066c) | 74092 | Multiplex 2 | 88 | [M13] - GACGGTCCGAATTGCCTTGG | GACCAGGAGAAGGCCATCAAAGAG |
phoT (Rv0820) | 913274 | 141 | GCAATCGCCGTGCAACC | [M13] - CTGCATGTTATGGGTGACGATGAC | |
Rv0095c | 105139 | Multiplex 3 | 94 | ATAACGTCGGGCACTGACAAAGAG | [M13]-TCCCGTATCAACTCGTAGGATCTGG |
Rv0197 | 232574 | 81 | CCACGGCGGGGACAAGAT | [M13] -AGAAAGGCGCCGCTGTAGG | |
qcrB (Rv2196) | 2460626 | Multiplex 4 | 120 | [M13] - GGGCTCGCAGCCAGACTTC | ATGATCACGGCGACCCAGAC |
leuB (Rv2995c) | 3352929 | 108 | [M13] - TCGACGTCCGGGTAGCATTC | GCGTCGCAAGCATCTGACATT | |
gyrA (Rv0006) | codon 95 | Simplex | 320 | CAGCTACATCGACTATGCGA | [M13] - GGGCTTCGGTGTACCTCAT |
Universal primer | |||||
[M13]: CGCCAGGGTTTTCCCAGTCACGAC |