Table 2 DNA sequence of oligonucleotide primer and probes sets used in qPCR screens for target species
Assay | Target | Forward primer | Probe | Reverse primer |
|---|---|---|---|---|
COT005 | Peptostreptococcaceae sp. COT-005 | CGTAACCGAGGAAATTTTTCGA | TGGAATCAGTTACGTTTAGTG | GCAGGTTGCCCACGTGTT |
COT033 | Peptostreptococcus sp. COT-033 | CGCGGTTGTGCTTAGTATTGAG | CACAACTGAGCGGCGG | TCCATGTGTATAGGGCAGGTTACC |
COT016 | Neisseria animaloris COT-016 | AACTGTCCGAAAGGATGGCTAA | ATATTCTCTGAGGAGGAAAG | CGCAAGGCCCGAAGGT |
COT192 | Porphyromonas sp. IJD1952 | CTTGCCTGATAGAAAGGGATAACC | TGAAAGTCGGACTAATAC | TCATGCAATAACCCAAGACCATA |
COT108 | Porphyromonas canoris COT-108 | ATGGCGACCGGCGGAT | CCCCTCTGACAGGTAAGTTGCATACGC | CTTGAAATACCATGCAGYATCTCAAG |
COT052 | Porphyromonas gulae II COT-052 | GTTGAAAGACGGACTAATACC | CCTTGCCCGRTCATGCAACCAAGCAAG | CATGCCTATCTTACAGCTATAAAT |
COT080 | Pasteurellaceae [G-2] sp. COT-080 | CCTTCGGGTTGTAAAGTTCTT | AAGGTATCAACTWTAATAGAGTTGGTAAWDGACGTTAT | TGCTGGCACGGAGTTAG |
COT084 | Odoribacter denticanis COT-084 | GGGTAACAGGCGGTAGCAATAC | ATGCAATCTACCTTTTACC | AAAGAAATGCATCGGGTATTAATCC |
COT107 | Globicatella sp. COT-107 | CGGAAGGAGAACTTGTTCTTTGGA | TGTTACTCACCCGTGCGCCACT | GGTATTAGCACTCGTTTCCCAGT |
COT036 | Lachnospiraceae sp. COT-036 | GAAGCRCGGGAAGCGGAAGT | CGTTACTCACCCGTCCGCCACT | CTTTTCCCTCYGTATCATGCGATAC |
COT044 | Peptococcus sp. COT-044 | CGCATAATATCTCTTTATYGCATGATAG | CTAAACGACAGCDCTAAGGCCGTCTTT | TACTGATCGTCGCCTTGGT |
COT064 | Filifactor sp. COT-064 | GGTGCGTAACGTGTGGGTAA | CCTTTGTCATGGGAATAA | TTCGGTATTAGCTGCTCTTTCAAG |
COT227 | Peptostreptococcus sp. COT-227 | GCGACTGATTTGATGCTTGC | CACCCGTCCGCCGCTCAACTTTCAT | AACTTTTCAGTATGTTATCCATGTGTA |
COT388 | Clostridiales sp. COT-388 | GGAAGAAGACTTCGGTCAACGGA | CGTTACTCACCCGTCCGCCACT | CATTTGGATGCCCATTCGGTATG |
COT029 | Tissierella sp. COT-029 | GAAGAACCTGCCTTTCACATAGGA | CGGGATTAATACCAAATGA | CCCCAAAAACATGCGATCTC |
COT306 | Chloroflexi sp. COT-306 | GAACGGGTGCAGCGATGT | TGTTACTCACCCGTGCGCCACT | TAATCTGAGACAGCTTATGCGGT |
COT280 | Conchiformibius steedae COT-280 | GGGATAACTTGCCGAAAGGTAA | ACCTCGCGTTATCCGAGCGGCC | TAGGCTTTTACCCCACCAACT |
Uni B | All genus | CTCCTACGGGAGGCAGCAG | CCAGCAGCCGCGGT | GGACTACCAGGGTATCTAATCCTGTT |
Uni B | CCTGCGGGAGGCAGC | TACCGGGGTATCTAATCCCGTT | ||
Uni B | CTTCTACGGAAGGCAGCAGTAG | ACTACCAGGGTATCTAGTCCTGTTCG | ||
Uni B | CTTCTACGGGAGGCAGCAG | GACTACCAGGGTGTCTAATCCTGTT | ||
Uni B | CGACGGGAGGCAGCAG | GACTACTAGGGTATCTAATCCTGTTTGC | ||
Uni B | CTACGGGAGGCGGCAG | GACTACCAAGGTATCTAATCCTGTTTGC | ||
Uni B | GACTACCAGGGAATCTAATCCTGTTT | |||
Uni B | GACTATCAGGGTACCTAATCCTGTTTG | |||
Uni B | GACTACCGGAGTATCTAATTCCGTTC | |||
Uni B | GACTACCAGGGTATCTAATCCCGTT | |||
Uni B | CGGGGCATCTAATCCCGT |