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Table 5 Bacterial strains used in this study

From: Characterization and regulation of the Resistance-Nodulation-Cell Division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora

Strain

Relevant characteristics or genotypea

Reference or source

Escherichia coli

  

XL1-Blue

recA 1 endA 1 gyrA 96 thi-1 hsdR 17(rK − mK +) supE 44 relA 1 lac [F’ proAB lacI q ZΔM15Tn10(Tcr)]

Stratagene

BL21(DE3)

F− ompT gal dcm lon hsdS B(rB − mB −) λ(DE3)

Novagen

S17-1

Tcr Smr, recA pro hsdR (RP4-2-Tc::Mu-Km::Tn7)

[45]

S17-1 λ-pir

λpir phage lysogen of S17-1

[45]

DH5α λ-pir

F− supE 44 Δlac U169 (ΦlacZ ΔM15) recA 1 endA 1 hsdR 17(rK − mK +) thi-1 gyrA 96 relA 1, λpir phage lysogen

D. Lies, Caltech

Erwinia amylovora

  

Ea1189

wild type

GSPBb

Ea1189-3

Ea1189, Kmr cassette in acrB

[7]

Ea1189.ΔmdtABC

Ea1189, mdtABC deletion mutant

This study

Ea1189.ΔmdtUVW

Ea1189, mdtUVW deletion mutant

This study

  1. aAntibiotic resistance: Apr, ampicillin; Cmr, chloramphenicol; Kmr, kanamycin; Smr, streptomycin; Tcr, tetracycline.
  2. bGSPB, Göttinger Sammlung Phytopathogener Bakterien, Göttingen, Germany.