Figure 3

Comparison of the Colony-live system with the conventional method. SKO mutants in the Keio collection showing growth defects were statistically identified. Growth values were determined by either the conventional method (CONV) or MGR-mass* value of Colony-live system. (A) Observed growth defects of SKO mutants in each subset were confirmed by referring to cited literature or through additional experiments. (B) SKO mutants displaying significantly slow growth (yellow) that are disrupted in the cysteine biosynthesis pathway are shown. Genes with an asterisk (such as CysK*) indicate SKO mutants showing normal MGR but low SPG (see Figure 4). Metabolic reaction (solid lines), functional unit (boxes), and inner membrane (grey boxes) are shown. OAS: O-acetylserine.