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Table 1 Identified proteins

From: Comparing the different morphotypes of a fish pathogen - implications for key virulence factors in Flavobacterium columnare

Band name

Size (kDa) on gel

Protein identification by nanoLC-ESI-MS/MS

Match to ORF [Accession number in NCBI]

Best hit in BLAST: identity % (query cover %)

Predicted size (kDa)

1

≈ 55

Hypothetical protein

FCOL_07765 [YP_004942163.1]

FCOL_11410 47% (100%)

53.5

2

≈ 55

OmpA family outer membrane protein P60

FCOL_09105 [YP_004942423.1]

FP0156 Outer membrane protein precursor; OmpA family P60 [F. psychrophilum JIP02/86] 70% (100%)

50.2

3

45

Hypothetical protein

FCOL_02860 [YP_004941210.1]

FP1486 Protein of unknown function [F. psychrophilum JIP02/86] 55% (98%)

44.5

4

35

Hypothetical protein

FCOL_08865 [YP_004942378.1]

FP0017 Putative cell surface protein precursor SprF [F. psychrophilum JIP02/86] 68% (94%)

37.9

5

20

Hypothetical protein

FCOL_11765 [YP_004942947.1]

FP2260 Protein of unknown function precursor [F. psychrophilum JIP02/86] 48% (100%)

24.4

ECP

13

Hypothetical protein

FCOL_04265 [YP_004941480.1]

-

18,1

  1. Protein identification of the membrane vesicle contents and the extracellular protein of Flavobacterium columnare B067 by nanoLC-ESI-MS/MS, and the subsequent identification by BLAST search. ORF annotation refers to F. columnare ATCC 49512 complete genome [NCBI: NC_016510].