Figure 3

Infection assays of Francisella strain W12-1067 and L. pneumophila Paris (Lpp) using the J774A.1 mouse cell line. Growth curve over a period of four days. Cells were infected with bacteria at an MOI of 10. Cells were washed three times with RPMI and incubated with 50 μg/ml of Gentamycin for 1 h to kill extracellular bacteria. Cells were washed again three times with RPMI and covered with 1 ml of RPMI + 10% FCS. The number of CFU per well was determined by plating on CHA agar plates. Results are mean standard deviations of three independent experiments of duplicate samples.