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Table 1 Comparison of recombinant plasmids with wildtype pCT plasmid

From: Functional genomics to identify the factors contributing to successful persistence and global spread of an antibiotic resistance plasmid

Gene inactivated on pCT

Stability

Conjugation to an E. coli recipient

Conjugation to a Salmonella recipient

Bacterial host growth kinetics

Biofilm formation

Competitive index when co-cultured with WT pCT

Sigma factor::aph

=

=

=

=

=

1.00

pilS::aph

=

=

=

1.00

traY::aph

=

UD

UD

=

=

0.99

rci::aph

=

=

=

=

0.99

pndACB::aph

=

=

=

=

=

1.00

parB::aph

=

ND

ND

=

=

ND

  1. =, the same as wild-type (WT) pCT; , reduced rate when compared to pCT; ND, not determined; UD, Undetectable.