Figure 6

Growth of P. falciparum co-cultured with PfRBCs and RBCs that were pretreated separately with the chelators. Synchronized PfRBCs at the ring stage and RBCs were treated with graded concentrations of Neocuproine and/or Cuprizone for 0.5 h or 2.5 h at room temperature. After washing, both treated RBCs and PfRBCs were mixed (pretreated PfRBCs plus non-treated RBCs (a) or non-treated PfRBCs plus pretreated RBCs (b, c)) at a ratio of more than 10 times RBCs to PfRBCs, and cultured in GFSRPMI (b) or CDRPMI (a, c) for 95 h. RBCs were pretreated for 2.5 h (b, c); (*) indicates a significant difference versus no treatment with Neocuproine and/or Cuprizone. (N + C) indicates the mixture of Neocuproine and Cuprizone (1:1).