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Figure 2 | BMC Microbiology

Figure 2

From: Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection

Figure 2

Mutagenesis of ompP4 . A, Composite gel of the ompP4 locus amplified using primers that flank the ompP4 ORF. Lane 1, standard; lane 2, 35000HPompP4; lane 3, 35000HP. B, Composite Southern blot of 35000HPompP4 and 35000HP probed with the cloned ompP4 insert (lanes 1, 2) or the kan cassette (lanes 3, 4). Lanes 1 and 4, 35000HPompP4; lanes 2 and 3, 35000HP. C, SDS-PAGE and Coomassie blue staining of OMPs prepared from 35000HPompP4 (lane 2) and 35000HP (lane 3); molecular markers are shown in lane 1, with sizes indicated to the left of the panel. Arrow points to the 30 kDa protein, the predicted size of OmpP4, missing in the ompP4 mutant.

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