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Table 3 Bacterial strains and plasmids used in this work

From: The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen

Strain or plasmid

Relevant characteristics

Source or reference

Strains

  

  C. defragrans

 

[21]

  65Phen-RIF

RaR

[27]

  ΔgeoB

65Phen-RIF, RaR, ΔgeoB

This study

  ctmA::Tn5a

65Phen-RIF, RaR, KmR, transposon insertion in ctmA at position 133

This study

  ctmA::Tn5b

65Phen-RIF, RaR, KmR, transposon insertion in ctmA at position 1188

This study

  ctmB::Tn5a

65Phen-RIF, RaR, KmR, transposon insertion in ctmB at position 38

This study

  ctmB::Tn5b

65Phen-RIF, RaR, KmR, transposon insertion in ctmB at position 559

This study

  ctmE::Tn5

65Phen-RIF, RaR, KmR, transposon insertion in ctmE at position 111

This study

E. coli

  

  W20767

RP4-2-tet::Mu-1, kan::Tn7 integrant, leu-63::IS10, recA1, creC510, hsdR17, endA1, zbf-5, uidA, (ΔMluI)::pir + thi

[37]

  S17-1

Thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7]

[38]

Plasmids

  

  pRL27

Tn5 with KmR, R6K ori, oriT, RP4, tnp

[31]

  pCR4-TOPO

AmR, KmR, lacZα

Invitrogen

  pK19mobsacB

KmR, mob, sacB modified from B. subtilis, lacZα

[39]

  pK19mobsacBΔgeoB

KmR, mob, sacB modified from B. subtilis, lacZα, 2000b flanking regions of ΔgeoB

This study