From: The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen
Inactivated gene | Annotation | Length [b] | Insertion positions [b] | Growth substrate* | |||
---|---|---|---|---|---|---|---|
Acetate | Limonene | β-Myrcene | Further substrates | ||||
Initial oxidation | |||||||
CDM25290 CtmA | Limonene dehydrogenase, alpha subunit | 1698 | 133 | + | - | - | Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-) |
CDM25290 CtmA | Limonene dehydrogenase, alpha subunit | 1698 | 1188 | + | - | - | Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-) |
CDM25289 CtmB | Limonene dehydrogenase, beta subunit | 1650 | 38 | + | - | - | Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-) |
CDM25289 CtmB | Limonene dehydrogenase, beta subunit | 1650 | 559 | + | - | - | Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-) |
CDM25286 CtmE | Ferredoxin, 2Fe-2S | 324 | 111 | + | - | - | Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-) |
Ring cleavage and β-oxidation | |||||||
CDM23589 | Electron transfer flavoprotein:ubiquinone oxidoreductase | 1647 | 138 | + | (+) | (+) | Perillic acid ((+)) |
CDM25250 MrcF | Perillyl-CoA hydratase | 1239 | 18 | + | - | - | α-phellandrene (-) |
CDM25253 MrcC | 2,4-dienoyl-CoA reductase | 888 | 841 | + | - | - | α-phellandrene (-) |
CDM25297 | Hypothetical protein | 1023 | 34 | + | - | - | |
CDM258488 | Electron transfer protein | 888 | 385 | + | - | - | Perillic acid (-) |
CDM25923 | Enoyl-CoA hydratase | 777 | 461 | + | - | - | |
Methylcitrate cycle | |||||||
CDM25082 | 2-methylcitrate dehydratase | 1452 | 262 | + | - | - | |
CDM25081 | 2-methylcitrate synthase | 1239 | -18 | + | (+) | + | |
Other functions | |||||||
CDM23676 | Molybdenum transport system protein | 865 | 47 | + | - | - | Perillyl aldehyde (-) |
CDM25260 | RND efflux transporter, periplasmic component | 1356 | 24 | + | (+) | (+) | Perillic acid ((+)) |