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Table 2 Growth of Castellaniella defragrans 65Phen transposon insertion mutants in liquid medium

From: The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen

Inactivated gene

Annotation

Length [b]

Insertion positions [b]

Growth substrate*

Acetate

Limonene

β-Myrcene

Further substrates

Initial oxidation

CDM25290 CtmA

Limonene dehydrogenase, alpha subunit

1698

133

+

-

-

Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)

CDM25290 CtmA

Limonene dehydrogenase, alpha subunit

1698

1188

+

-

-

Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)

CDM25289 CtmB

Limonene dehydrogenase, beta subunit

1650

38

+

-

-

Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)

CDM25289 CtmB

Limonene dehydrogenase, beta subunit

1650

559

+

-

-

Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)

CDM25286 CtmE

Ferredoxin, 2Fe-2S

324

111

+

-

-

Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)

Ring cleavage and β-oxidation

CDM23589

Electron transfer flavoprotein:ubiquinone oxidoreductase

1647

138

+

(+)

(+)

Perillic acid ((+))

CDM25250 MrcF

Perillyl-CoA hydratase

1239

18

+

-

-

α-phellandrene (-)

CDM25253 MrcC

2,4-dienoyl-CoA reductase

888

841

+

-

-

α-phellandrene (-)

CDM25297

Hypothetical protein

1023

34

+

-

-

 

CDM258488

Electron transfer protein

888

385

+

-

-

Perillic acid (-)

CDM25923

Enoyl-CoA hydratase

777

461

+

-

-

 

Methylcitrate cycle

CDM25082

2-methylcitrate dehydratase

1452

262

+

-

-

 

CDM25081

2-methylcitrate synthase

1239

-18

+

(+)

+

 

Other functions

CDM23676

Molybdenum transport system protein

865

47

+

-

-

Perillyl aldehyde (-)

CDM25260

RND efflux transporter, periplasmic component

1356

24

+

(+)

(+)

Perillic acid ((+))

  1. * + growth like wild type; - no growth; (+) decreased growth rate and maximum density compared to wild type.