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Figure 3 | BMC Microbiology

Figure 3

From: Bdellovibrio bacteriovorus HD100 guards against Pseudomonas tolaasii brown-blotch lesions on the surface of post-harvest Agaricus bisporus supermarket mushrooms

Figure 3

Predatory interactions between Bdellovibrio and P. tolaasii in funga” on the mushroom pileus surface. Scanning Electron Microscope images showing the mushroom pileus surface 48 hours after the following treatments: a. untreated mushroom pileus surface b. inoculation of P. tolaasii 2192T alone c. Inoculation of B. bacteriovorus HD100 alone d. and e. Co-inoculation of P. tolaasii 2192T and B. bacteriovorus HD100 and f. Application of King’s medium B alone. In image e, B. bacteriovorus HD100 (blue) are shown attached at one pole to P. tolaasii 2192T (yellow), a crucial first step in the predatory process. Images d and e both show rounded P. tolaasii 2192T cells, characteristic of the bdelloplast structures formed after Bdellovibrio invades the host cell and begins replication. 1 μm scale bar shown.

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