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Table 1 Bacterial strains and plasmids used in this study

From: The Pseudomonas aeruginosa rhlG and rhlAB genes are inversely regulated and RhlG is not required for rhamnolipid synthesis

Strain or plasmid

Description

Reference(s) or source

P. aeruginosa

  

PAO1

Plasmid-free strain

[31]

PAO6358

rpoN mutant

[24]

PDO100

rhlI mutant

[25]

PAOGAB

rhlG mutant

This study

PAOFDO

PA3388 mutant

This study

PAOJBB

rhlG/PA3388 mutant

This study

PAOU

algU mutant

[21]

Escherichia coli

  

Top10

Electrocompetent cells

Invitrogen

S17.1

RecA pro (RP4-2Tet::Mu Kan::Tn7)

[26]

Plasmids

  

pBBR1MCS-5

Cloning vector, GmR

[32]

pAB133

Promoter-less luxCDABE operon cloned in pBBR1MCS-5, GmR

[17]

pAB134

rhlG promoter cloned in pAB133, GmR

This study

pEX100Tlink

P. aeruginosa suicide vector, AmpR

[26]

pUCGmlox

AmpR, GmR, pUC18-based vector containing the lox flanked aacC1

[26]

pCM157

cre expression vector, TcR

[33]

pGAB10

Deleted rhlG cloned in pEX100Tlink, AmpR

This study

pFAB1

Deleted PA3388 cloned in pEX100Tlink, AmpR

This study

pJBB1

Deleted rhlG-PA3388 operon cloned in pEX100Tlink, AmpR

This study

pGAB10.14

lox flanked aacC1 from pUCGmlox cloned in pGAB10, AmpR GmR

This study

PFAB1.13

lox flanked aacC1 from pUCGmlox cloned in pFAB1, AmpR GmR

This study

pJBB11

lox flanked aacC1 from pUCGmlox cloned in pJBB, AmpR GmR

This study

pGAB

Complementation, rhlG cloned in pBBR1MCS-5, GmR

This study