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Figure 7 | BMC Microbiology

Figure 7

From: The Escherichia coli uropathogenic-specific-protein-associated immunity protein 3 (Imu3) has nucleic acid -binding activity

Figure 7

Representative electromobility shift assays of re-ligated DNA previously complexed with Imu3 (0.8% agarose gels). Lane 1, 100 ng pUC19/Eco RI DNA; lane 2: 100 ng pUC19/Eco RI DNA purified with the QIAprep kit; lane 3: 100 ng pUC19/Eco RI DNA–Imu3 complex purified with the QIAprep kit; lane 4: ligation reaction of purified DNA; lane 5: ligation reaction of purified DNA–Imu3 complex; lane 6: restriction (Eco RI) of ligation reaction of purified DNA (from lane 4); lane 7: restriction (Eco RI) of ligation reaction of purified DNA–Imu3 complex (from lane 5). M: λ/Pst I marker.

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BMC Microbiology

ISSN: 1471-2180

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