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Table 3 Theoretical and measured masses of OmpUs of 16 V. cholerae isolates

From: OmpU as a biomarker for rapid discrimination between toxigenic and epidemic Vibrio cholerae O1/O139 and non-epidemic Vibrio cholerae in a modified MALDI-TOF MS assay

Isolate

GT

 

Theoretical

  

Measured

 
   

1stexp

 

2ndexp

massa

Δb

massc

Δ refd

massc

Δ refd

080025/EZ

1

34656

0

34755

+ 6

34567

+ 12

FFIVC130

1

34656

0

34742

- 6

34543

- 12

FFIVC129

1

34657

+ 1

N.D.e

 

N.D.e

 

FFIVC114

4

35595

+ 939

35683

+ 934

35506

- 951

080025/FE

2

34584

- 72

34672

- 77

34482

- 73

080025/FI

2

34584

- 72

34678

- 71

34508

- 47

080025/FL

3

35566

+ 910

35656

+ 907

35469

+ 914

17/110/2006

6

33871

- 785

33975

- 774

33733

- 822

2/110/2006

5

34961

+ 305

35031

+ 282

34875

+ 320

080025/FR

singleton

34870

+ 214

34951

+ 203

34784

+ 229

080025/GE

3

35566

+ 910

35670

+ 922

35501

+ 946

FFIVC050

singleton

33840

- 816

33924

- 824

33748

- 807

FFIVC084

singleton

34811

+ 155

34884

+ 136

34683

+ 128

FFIVC137

singleton

35709

+ 1053

35813

+ 1065

N.D.f

 

4/110/2006

singleton

34122

- 534

34198

- 550

33977

- 578

14/110/2006

singleton

34826

+ 170

N.D.f

 

34716

+ 161

  1. aTheoretical mass of mature OmpU in Da.
  2. bDifference in mass with theoretical mass of OmpU of isolate 080025/EZ, in Da.
  3. cMean of peak masses obtained from 4 different MALDI spots.
  4. dThe average of OmpU peak masses of strain 080025/EZ and FFIVC130 was set as reference.
  5. eN.D.: not determined, as OmpT instead of OmpU was assigned as the major peak in the 30000 – 40000 m/z range.
  6. fN.D.: not determined because of failed measurement.