Figure 3

Comparisons of recombination efficiency reflected by transduction frequencies, with the points representing the numbers of transductants. A, Transduction of ompD 159 between S. gallinarum and S. pullorum (see text for more details). Groups: 1, S. pullorum recipients and S. pullorum DNA; 2, S. pullorum recipients and S. gallinarum DNA; 3, S. gallinarum recipients and S. gallinarum DNA; 4, S. gallinarum recipients and S. pullorum DNA. Note that group 3 has the largest numbers of recipients (groups 2 vs 3, p = 0.007; groups 3 vs 4, p = 0.000), reflecting significantly higher recombination efficiency of S. gallinarum DNA into the S. gallinarum genome (group 3) than S. gallinarum DNA to the S. pullorum genome (group 2) or than S. pullorum DNA to the S. gallinarum genome (group 4). Group 1 had a similar tendency as group 3, although the differences were not statistically significant (groups 1 vs 2, p = 0.079; groups 1 vs 4, p = 0.071). B, Transduction with S. pullorum as the recipient and S. pullorum or S. gallinarum as the donor. Groups: 1, S. pullorum recipients with DNA fromS. gallinarum 287/91; 2, S. pullorum recipients with DNA from S. pullorum RKS5078 (groups 1 vs 2, p = 0.009). C, Transduction with S. pullorum or S. gallinarum strains as the recipient and donor DNA from S. gallinarum. Groups: 1, S. pullorum recipients with DNA from S. gallinarum strain 287/91; 2, S. gallinarum recipients with DNA from S. gallinarum strain 287/91 (groups 1 vs 2, p = 0.000).