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Figure 5 | BMC Microbiology

Figure 5

From: The genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon Tnces in emetic Bacillus weihenstephanensis

Figure 5

Southern blot hybridization analysis of the transconjugants of Tn ces :: km transposition in E. coli HB101. Two independent hybridizations were performed. A: lane 1–4, independent KmRSmR transconjugants, lane 5, HB101, lane 6, JM109 (pTnkm); and B: lane 1–5, independent KmRSmR transconjugants, lane 6, HB101, lane 7, JM109 (pTnkm). Three probes of Km (a), ISces (b) and blapuc18 (c), respectively were used for hybridization as illustrated in Figure 4.

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