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Table 2 The methyl viologen-dependent (MV + ) nitrate reductase (MV + -NR), nitrite reductase (MV + -Nir) and nitric oxide reductase (Nor) activities of E. meliloti 1021 (WT) and the napA, nirK , and norC mutant strains incubated in MMN under 2% initial O 2 or anoxic conditions

From: Genetic basis for denitrification in Ensifer meliloti

Strain

Genotype

Oxygen conditions

2% O2

Anoxia

  

MV+-NRa

MV+-NiRb

Norc

MV+-NR

MV+-NiR

Nor

1021

WT

210.93 (10.33)

32.57 (1.42)

563.33 (21.81)

62.96 (5.70)

10.522 (1.465)

335.88 (32.12)

STM.3.02.F08

napA

18.86 (3.79)

-

-

n.d.

-

-

STM.1.13.B08

nirK

-

3.34 (0.26)

528.26 (20.86)

-

n.d.

308.19 (23.18)

G1PELR32E8

norC

-

-

1.11 (0.01)

-

-

2.84 (0.78)

  1. aMV+-NR and bMV+-Nir activities are expressed as nmol NO2 - produced or consumed · mg protein-1 · min-1. Nor activity is expressed as nmol NO consumed · mg protein-1 · min-1. All of the activities were determined after incubation for 18 h. The data are expressed as the means with the standard error in parentheses from at least two different cultures assayed in triplicate. -, not determined; n.d., not detectable.