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Figure 3 | BMC Microbiology

Figure 3

From: Identification of natural antimicrobial agents to treat dengue infection: In vitro analysis of latarcin peptide activity against dengue virus

Figure 3

Effect of the Ltc 1 peptide on cells proliferation and viral replication in HepG2 cells. (A) The cytotoxic effect of the Ltc 1 peptide on cell viability was measured by non-radioactive cell proliferation assay. (B) The effect of the Ltc 1 peptide on cell proliferation was measured with the xCELLigence Real-Time Cellular Analysis (RTCA) system. Cell viability and growth were monitored continuously after applying increasing concentrations of the Ltc 1 peptide (0 (cyan), 12.5 (purple), 25 (dark green), 50 (magenta), 100 (orange), 150 (blue), 200 (green), and 250 μM (red)). (C) The effect of the Ltc 1 peptide on virus replication in infected cells. Viral particles were labelled with FITC fluorescence dye using indirect immunostaining, and the cell nuclei were stained with Hoechst. The figure shows a significant reduction of viral particles after peptide treatment. (D) Western blot analysis of the DENV2 NS1 protein expression level normalised to beta-actin as a reference cell protein (L1, untreated control; L2, DENV2-infected cells treated with Ltc 1 peptide).

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