Table 1 Primers used in this study
From: RpoS integrates CRP, Fis, and PhoP signaling pathways to control Salmonella Typhi hlyE expression
Primers used for the red/swap technique [[44]] | |
|---|---|
crp (H1 + P1) | TTCTTGTCTCATTGCCACATTCATAAGTACCCGTCAAAGAtgtaggctggagctgcttcg |
crp (H2 + P2) | GGCGAGGTTACCTACTTTTTCAGAGGTGACTTGTAAGCGAcatatgaatatcctccttag |
fis (H1 + P1) | AAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGtgtaggctggagctgcttcg |
fis (H2 + P2) | ACGCAGCGTACCACGGTTGATGCCCATCATCAGAGCAGCAcatatgaatatcctccttag |
rpoS (H1 + P1) | AATACGCTGAAAGTTCATGATTTAAATGAAGACGCGGGtgtaggctggagctgcttcg |
rpoS (H2 + P2) | GCGCAGGTATACGTTCAGCTCTTTAACAATGTGAATcatatgaatatcctccttag |
phoP (H1 + P1) | CCAGGATTCAGGTCACCAGGTCGATGCCGCAGAAGATGtgtaggctggagctgcttcg |
phoQ (H2 + P2) | TTCCTCTTTCTGTGTGGGATGCTGTCGGCCAAAAACGACCcatatgaatatcctccttag |
Primers for epitope tagging (3xFLAG) [22] | |
rpoS-3xFLAG | GCAGACGCAGGGGCTGAATATCGAAGCGCTGTTCCGCGAGgactacaaagaccatgacgg |
rpoS-kan | TCTGGACGGTATATCAGTGTCAGCATTGTCTGTATACCTGcatatgaatatcctccttag |
hlyE-3xFLAG | AAGACACGGTAAGAAGACGCTTTTCGAGGTTCCTGACGTCgactacaaagaccatgacgg |
hlyE-kan | GAATGCGGAAATCACCCTCGACTACCAGCTTAACGCCTGACcatatgaatatcctccttag |
Primers used for cloning | |
CRP-C | CTGCAG GCTGGCCTATCAATAAACCA |
CRP-N | CTGCAG AAGCGAGACACCAGGAGACA |
RPOS-C | CTGCAG CCGATGATTTGTCCACGCTG |
RPOS-N | CTGCAG TGCCCCGTATAGCCTGAATG |
PHOPQ-C | CGTTCAAGAAAGTCGGGCCA |
PHOPQ-N | GCCTTAAAGCCATGACGCCG |
FIS-C | TTCTGCAG GCGCCTTTTTAAACAAGCAG |
FIS-N | TTCTGCAG GACCAGTTTCGGCGCACATT |
Primers for mRNA detection by RT-PCR | |
RT-HLYE-C2 | CGCTTCATTCAGTTTCTTGA |
RT-HLYE-N | AAGTTTTGCTTATGGACAGC |
RT-RPOS-C | TTCGGAATCACCGCCCAGCG |
RT-RPOS-N | TCGCCGTCGCATGATTGAGAG |
RT-FIS-C | CATCATCAGAGCAGCACGGG |
RT-FIS-N | TCAGGATCAGGTAACCCAAA |
16SFW | GTAGAATTCCAGGTGTAGCG |
16SRV | TTATCACTGGCAGTCTCCTT |