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Table 3 Resistance genotypes, PBP3 types and PBP3 substitutions

From: Multilocus sequence typing and ftsI sequencing: a powerful tool for surveillance of penicillin-binding protein 3-mediated beta-lactam resistance in nontypeable Haemophilus influenzae

Resistance genotypesa

PBP3 typesb

n c

Sgd

Blae

PBP3 substitutionsf

D

S

A

M

S

P

A

I

G

A

V

R

N

A

T

V

D

A

N

350

357

368

377

385

392

437

449

490

502

511

517

526

530

532

547

551

554

569

High-rPBP3

                     

 Group III

-

1

  

N

N

  

T

    

T

  

K g

  

I

  

S

 Group III-like

-

2

  

N

N

 

I

T

      

H

  

S

I

   

Low-rPBP3

                     

 Group II

A

48

 

1

N

  

I

     

V

  

K h

  

I

  

S

 

B

19

 

5

       

V

    

K g

  

I

  

S

 

C

5

  

N

  

I

    

E

   

K h

  

I

  

S

 

D

17

  

N

       

E

   

K g

S

     
 

F

1

              

K g

      
 

H

6

           

V

  

K h

      
 

I

4

  

N

     

S

  

V

  

K g

  

I

  

S

 

J

3

  

N

        

T

  

K g

  

I

  

S

 

K

2

    

T

      

T

  

K g

      
 

L

1

  

N

       

E

   

K g

  

I

 

Di

S

 

M

1

  

N

        

V

  

K h

  

I

  

S

 

N

1

 

1

N

     

S

V

    

K g

  

I

  

S

 

O

1

           

T

  

K g

  

I

  

S

 

P

1

           

T

  

K g

  

I

   
 

Q

1

          

E

V

  

K h

  

I

  

S

 Group I

-

2

             

H

   

I

 

T

 

sPBP3

z0

51

15

6

                   

z4

9

1

 

N

              

I

  

S

z1

7

3

2

               

I

   

z6

3

                 

I

  

S

z7

3

                 

I

 

T

S

z5

1

 

1

         

S

         

z8

1

  

N

        

T

     

I

  

S

z9

1

  

N

              

I

   

z10

1

                 

I

Ai

  

z11

1

            

A

    

I

   

z12

1

       

Si

             

z13

1

           

T

         
  1. aSee Table 1.
  2. bPBP3 types according to Skaare et al.[11] (types A – G) and this study (types H – Q and z0 – z13). ‘-‘, not designated.
  3. c n, No. of study isolates.
  4. dSg, No. of isolates from the Susceptible group included in n.
  5. eBla, No. of beta-lactamase positive isolates (all TEM-1) included in n.
  6. fAmino acid substitutions in PBP3 (transpeptidase domain, 338–573) with the amino acid sequence of H. influenzae Rd KW20 [GenBank:U32793] as reference (z0). Essential substitutions in bold.
  7. gN526K encoded by the DNA triplet AAG.
  8. hN526K encoded by the DNA triplet AAA.
  9. iNovel substitution.