Table 2 New and modified primers and probes used in this study
Name | Function | Target | Sequences (5′ to 3′)a | Original (reference) |
|---|---|---|---|---|
bexA Fb | F-primer | bexA | CGTTTA TR TGATGTTGATCCT GA | HI-1 [35] |
bexA Rb | R-primer | bexA | TGTCCATA TCTTCAAAATGG TG | HI-2 [35] |
bexA P | Probe | bexA | FAM ATGCAAGYCGRGCTTTCATCCCTG-BHQ | This study |
Hinf_fR | R-primer | cap (serotype f) | GG TACTATCAAGTCCAAATC | f3 [35] |
Hinf_eR2 | R-primer | cap (serotype e) | CTAATTGTTCTTTCTGTCTA | This study |
ompP6 P | Probe | ompP6 | ACG TGG TAC ACC AGA ATA CAA CAT CGA | This study |
H16SP | Probe | 16S rRNA gene | TCGCTCCACCTCGCAGCTTCGCT | This study |
TEMP | Probe | bla TEM | CAG CTC CGG TTC CCA ACG ATC AAG | This study |
ROBP | Probe | bla ROB | TAG CGA CAA CAG CGC GAC CAA TTT G | This study |