Figure 4

MRSP biofilm surface height profiles with corresponding AFM deflection mode images (Scale = 5 μm). (A), (B) MRSP A12 AFM image showing clusters of biofilms with extended chains exhibiting stable nanoscale morphology. (C), (D) Fosfomycin treated MRSP biofilms for 4 h exhibits greater deviation in nanoscale morphology and reduced height indicating the efficacy of fosfomycin. The cellular ultrastructure has been significantly altered with less surface coverage and a smaller cell diameter.