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Figure 1 | BMC Microbiology

Figure 1

From: Mycobacterium tuberculosis expressing phospholipase C subverts PGE2 synthesis and induces necrosis in alveolar macrophages

Figure 1

Intracellular killing of Mtb isolates 97-1200 or 97-1505 and cell death of infected alveolar macrophages. Alveolar macrophages were infected in vitro for 24 h with Mtb isolates 97-1200 or 97-1505 at MOI 5. (A) Bacterial killing was assessed by resazurin metabolisation and expressed as a percentage of phagocytised bacteria. (B) Cell viability assessed by resazurin metabolisation. Maximum viability (100%) is based on uninfected cells. (C) ELISA assay of apoptosis and necrosis 24 h post-infection of alveolar macrophages in vitro. Camptothecin 5 μg/mL (CAMP) was used as apoptosis-positive control and hypertonic buffer as necrosis-positive control. # P < 0.0001 for uninfected cells vs. infected cells (97-1505 or 97-1200); ***P < 0.0001; **P < 0.001 (one-way ANOVA). Data are representative of three (A, B) and two (C) independent experiments (error bars, s.e.m.).

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BMC Microbiology

ISSN: 1471-2180

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