Table 1 MAMA real-time PCR assay sequences and targets for genotyping C. gattii
From: Real-time PCR assays for genotyping of Cryptococcus gattii in North America
Genotype | Assay Name | Gene (SNP position) | Base call match/mismatch | Universal Primer sequence 5′ -- > 3′ | Match MAMA Primer sequence 5′ -- > 3′ | Mismatch MAMA Primer sequence 5′ -- > 3′ |
|---|---|---|---|---|---|---|
VGI | VGI-MPD471 | MPD1 (471) | G/A | AGACTGTCCCAATGTCAAGCTTTC | GCCTTGTATGTGGTAACACCAGTG | GWGCCTTGTATGTGGTAACACCAGTA |
VGII | VGII-MPD495 | MPD1 (495) | T/A | AGACTGTCCCAATGTCAAGCTTTC | ATTAACCTTAGTGTTGGAGACCTTGACT | AACCTTAGTGTTGGAGACCTTGACA |
VGIIa | VGIIa-45211 | hypothetical protein | A/C | CCCAGCAACCTTGATCTGGA | AGCTGCTCTAAGAGACACATCATCA | AGCTGCTCTAAGAGACACATCATCC |
VGIIb | VGIIb-502129 | not annotated | G/A | AATCGCTCGTCCTCATATGACA | GTAGGCGGTGGGATAAGGTG | GGTAGGCGGTGGGATAAGGTA |
VGIIc | VGIIc-257655 | non-coding region | C/T | CGTTAATTTGGTTGTTTGACAACCT | AGCAACTCACGCAGAAACAGAC | GAGCAACTCACGCAGAAACAGAT |
VGIII | VGIII-MPD198 | MPD1 (198) | T/A | TGACATTGGGACAGTCTGCAAT | ACTGCTGCTTCTCCCGTTGT | CTGCTGCTTCTCCCGTTGA |
VGIV | VGIV-MPD423 | MPD1 (423) | A/C | ACCCAGTCATTAACCTTAGTGTTGGA | CTCGTTCGTCAAYCACGTTAGA | TCGTTCGTCAAYCACGTTAGC |