Table 3 List of primers for this study
Primer name | Sequences (5′-3′) |
|---|---|
Mutant construction | |
BsaM up for | AAGCTTCACGCGACGCGATTTTGAATTG |
BsaM up rev | AAGCTTGCTCGCCGACGCAGAAAAATA |
BsaM dn for | GAATTCAAGCTTGATCACGCGTCCTGGTATTT |
BsaM dn rev | TTGGATCCAAGCGAGACGTAGATGCTG |
BopA up for | CCAAGCTTGCATGCCTGCAGGTCTTGCTCTCGGTTGAAGG |
BopA up rev | GAGGATCCCCGGGTATGCATCGACATTGATCATCC |
BopA dn for | TACCCGGGGATCCTCGCATGAAGAACGCATGAAGA |
BopA dn rev | CCATGATTACGAATTCGATTCTTGTTGCTCCGATGC |
BopC up for | CCATGATTACGAATTCCCCGACCAGTTGAAGATGTC |
BopC up rev | GAGGATCCCCGGGTAGAACCAATGCCTAGCCTCAC |
BopC dn for | TACCCGGGGATCCTCCTGGGGTCGGTTTACATACG |
BopC dn rev | CCAAGCTTGCATGCCTGCAGGAGCATCGCGAATACGAACT |
BopE up for | CGGTACCCGGGGATCCAACAACCGCTCCTTCATCC |
BopE up rev | TCATGTCTTGCTCTCGGTTG |
BopE dn for | GAGAGCAAGACATGAGACGCTCGAAGCCACATAC |
BopE dn rev | GGCCAGTGCCAAGCTTGTATTACGAGTCGGGGCTGA |
Bt BsaM up for | GGCCAGTGCCAAGCTTTTTCCAGAAAAGCGAGCAAT |
Bt BsaM up rev | GGCGATAAATGGCCTGATTA |
Bt BsaM dn for | AGGCCATTTATCGCCGATCACGCGTCCTGGTATTT |
Bt BsaM dn rev | CGGTACCCGGGGATCCAGCAGCGAGAAAGAAACGAA |
Expression in mammalian cells | |
BopA for | GAGGAGTGGATGATCAATGTCG |
BopA rev | GTTCTTCATGCTGTCTTCAGGC |
BopC for | AAGCTTAAGATGCCGAGCATGACC |
BopC rev | GGATCCTCATGCGAGTGGGGTGTC |
Real-Time PCR | |
BopA RT for | TTCGTGCTGTTCGGGCTGA |
BopA RT rev | TCGATACTTGAGCTCGCCTGACTT |
BopC RT for | TACCGACGATCTCGTCAAAG |
BopC RT rev | GCGTTCAAGGAAGTTAAGCC |
BopE RT for | TCCTTCGCTTCGCTGAAGATCG [18] |
BopE RT rev | ATTCGGCCGGCAAGTCTACG [18] |
GAPDH for | CAATGACCCCTTCATTGACC [44] |
GAPDH rev | GTTCACACCCATGACGAACATG [44] |