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Figure 6 | BMC Microbiology

Figure 6

From: Functional characterization of a Penicillium chrysogenum mutanase gene induced upon co-cultivation with Bacillus subtilis

Figure 6

Mutanase production by P. chrysogenum after addition of cell-free supernatants from mixed cultures of P. chrysogenum and B. subtilis . Mutanase activity was measured after addition of supernatant samples of mixed cultures of P. chrysogenum and B. subtilis grown in shake flasks (), of a 24-h mixed chemostat culture () to a 24-h batch culture of P. chrysogenum and in culture grown in presence of 0.1% of mutan (α 1,3 glucan) (▲), of 0.1% of pullulan (α-1,4-α-1,6-glucan), of 0.1% of dextran (α1,6 glucan) (×) and 0.1% of β1,3 glucan (∆) The presented data are averages and standard deviations of triplicate experiments. We It is worth mentioning that the residual mutanase activity (0.020 U.ml-1) observed at the start of the P. chrysogenum shake flask cultures spiked with supernatant of mixed culture originated from the inoculum. Data are presented as average ± mean deviation of three independent experiments.

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