Table 1 Primers used in the study
Loci | Primers | Sequences | Annealing T° (time) | Expected size | Reference |
|---|---|---|---|---|---|
katG | F | 5′-GAAACAGCGGCGCTGATCGT-3′ | 66°C (1 min) | 210 bp | [21] |
R | 5′- GTTGTCCCATTTCGTCGGGG- 3′ | ||||
fabGI-inhA | F | 5′-CCTCGCTGCCCAGAAAGGGA-3′ | 64°C (1 min) | 248 bp | [21] |
R | 5′-ATCCCCCGGTTTCCTCCGGT-3′ | ||||
inhA (ORF) | F | 5′- GAACTCGACGTGCAAAAC – 3′ | 55°C (45 sec) | 207 pb | [18] |
R | 5′- CATCGAAGCATACGAATA – 3′ | ||||
ahpC | F | 5′-ACCACTGCTTTGCCGCCACC-3′ | 65°C (1 min) | 237 bp | [21] |
R | 5′-CCGATGAGAGCGGTGAGCTG-3′ | ||||
rpoB | F | 5′-TCGCCGCGATCAAGGAGT-3′ | 62°C (30 sec) | 158 bp | [21] |
R | 5′-GTGCACGTCGCGGACCTCCA-3′ | ||||
rrs530 | F | 5′-GATGACGGCCTTCGGGTTGT-3′ | 62°C (1 min) | 238 bp | [12] |
R | 5′- TCTAGTCTGCCCGTATCGCC -3′ | ||||
rrs912 | F | 5′- GTAGTCCACGCCGTAAACGG -3′ | 62°C (1 min) | 240 bp | [12] |
R | 5′- AGGCCACAAGGGAACGCCTA -3′ | ||||
rpsL | F | 5′- GGCCGACAAACAGAACGT -3′ | 58°C (30 sec) | 375 bp | [12] |
R | 5′- GTTCACCAACTGGGTGAC -3′ | ||||
embC | F | 5′- GTTCGACAAGCGCGCCACAC -3′ | 65°C (45 sec) | 334 bp | [22] |
R | 5′- CGGAGGTAGATGGTAGCCGG -3′ | ||||
embA | F | 5′- GCCGGCTATGTAGCCAACTA -3′ | 65°C (45 sec) | 338 bp | [17] |
R | 5′- GACCGTTCCACCAACACC -3′ | ||||
embB | F | 5′- CCGACCACGCTGAAACTG -3′ | 65°C (45 sec) | 368 bp | [23] |
R | 5′- GTAATACCAGCCGAAGGGATCCT -3′ | ||||
gidB | F | 5′-CGCCGAGTCGTTGTGCT-3′ | 62°C (1 min) | 886 pb | - |
|  | R | 5′-AGCCTGGCCCGACCTTA-3′ |  |  |  |