Table 2 Primers used in this study
From: L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences
Primer | Sequence | Application | Amplicon size |
|---|---|---|---|
A7F | 5′- GGATTTGGGATACCGCTCTT -3′ | gerA detection/sequencing | 718 bp |
A7R | 5′- TGCAGATGCTGCGAGAATAC -3′ | gerA detection/sequencing | 718 bp |
gerAAF MW3 | 5′- CCCTGTTCCTATCGGCGTTT -3′ | RT-PCR (E = 2.01) | 59 bp |
gerAAR MW3 | 5′- TCGGCAGCATGCCTTGA -3′ | RT-PCR (E = 2.01) | 59 bp |
gerAAF 1112/1032/800 | 5′- CGCCGTTCCCACAGATTC –3′ | RT-PCR (E = 2.01/1.98/1.95) | 55 bp |
gerAAR 1112/1032/800 | 5′- CAGCGCTGAAGAAACCTTGTC –3′ | RT-PCR (E = 2.01/1.98/1.95) | 55 bp |
rpoBF | 5′- ACCTCTTCTTATCAGTGGTTTCTTGAT -3′ | RT-PCR (E = 2.00) | 70 bp |
rpoBR | 5′- CCTCAATTGGCGATATGTCTTG -3′ | RT-PCR (E = 2.00) | 70 bp |