Figure 4
MMP-2 and −3 as well as TIMP-1 protein expression in P. gingivalis LPS- and E. coli LPS-treated HGFs. Confluent HGFs were stimulated with P. gingivalis (Pg) LPS1435/1449 (1 μg/ml), LPS1690 (1 μg/ml) and E. coli LPS (1 μg/ml) at 24 h and 48 h. Culture supernatants of 40 μg were subjected to SDS-PAGE and probed with anti-rabbit polyclonal MMP-2 (1:1000), MMP-3 (1:1000) and TIMP-1 (1:1000) antibodies. Blots were re-probed with α-Tubulin to confirm equal loading in samples. MMP-2: 64 kDa; MMP-3: 54 kDa; TIMP-1: 28 kDa and Tubulin: 50 kDa (a). Quantification of band intensities was performed by ImageJ software. The fold increase values of proteins MMP-2 (b), MMP-3 (c) and TIMP-1 (d) as compared with α-Tubulin are shown in the graphs. One representative blot was shown from three independent experiments. *Significant difference (p < 0.05) as compared with the data at 24 h.