The role of flagella and chemotaxis genes in host pathogen interaction of the host adapted Salmonella enterica serovar Dublin compared to the broad host range serovar S.Typhimurium

Table 2 Uptake and survival of S. Dublin 3246 (SDu) and S. Typhimurium (STm) wildtype and flagella and chemotaxis mutants in cultured J774A.1 macrophages ^a

Strain	Uptake 3h (Percent of wild type strain)	Survival 24 h (Percent of same strain at 3h)	Survival 48 h (Percent of same strain at 3 h)
SDu WT	100	124,1 ± 43.5	20.7 ± 4.7
SDu cheA	53.9 ± 15.1**	279.8 ± 65.8	53.8 ± 16.5
SDu cheB	1.4 ± 1.0**	307.7 ± 90.2	248.8 ± 39.8
SDu flic	1.0 ± 0.2***	450.5 ± 255.0	615.3 ± 325.8
STm WT	100	114.0 ± 42.6	2.8 ± 1.72.8
STm cheA	72.4 ± 22.4	100.2 ± 31.0	12.2. ± 3.1
STm cheB	19.0 ± 9.3**	309.8 ± 231.5	81.7 ± 6.9*
STm fliC/flijB	0.2 ± 0.1***	490.9 ± 111.6*	702.9 ± 53.0***

a: Uptake of mutant strains was expressed relatively to and compared statistically to the wild type strain of the same serovar. Survival at 24 and 48 hours was expressed relatively to the number of bacteria determined at 3 hours and compared statistically to the survival capability of the wild type strain of the same serotype. *: p<0.05; **: p<0.01 ***: p<0.001.

ISSN: 1471-2180