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Table 1 Strains and plasmids

From: Production of capsular polysaccharide does not influence Staphylococcus aureusvancomycin susceptibility

Strain/plasmid

Relevant genotype or phenotypea

Source/reference

S. aureus strains

  

SA137/93A

Clinical hVISA isolate; METr, Northern German epidemic MRSA, MLST sequence type ST247

[4]

SA137/93G

Mutant VISA of SA137/93A; ΔSCCmec (METs), ST247

[4, 27]

SA137/93G pCapDvorne

SA137/93G carrying pCapDvorne (CHLr)

This work

Mu50

Clinical VISA isolate

[29]

SA1450/94

Northern German epidemic MRSA, Iberian clone, ST247, CP5 positive, VANs control

German reference centre for staphylococci, Wernigerode

SA1450/94 pCapAre

SA1450/94, repair of cap5A

This work

SA1450/94 pCU1

SA1450/94, control (empty vector)

This work

Newman

CP5 positive, VANs

NCTC8178

Newman-132

pMUTIN integrated into capsule promoter

This work

NCTC8325 (RN1)

laboratory strain

National collection of type cultures

HG001

NCTC8325, repaired in rsbU

[31]

HG001 pCap5E

HG001 capsule production repaired

This work

SG511-Berlin

sensitive antibiotic screening strain

[32]

RN4220

Restriction negative derivative of NCTC8325, cloning host

[33]

Plasmids

  

pUC19gyrB

pUC19, carrying a 560 bp internal fragment of gyrB; external plasmid standard for real time PCR

This work

pCU1cap5E

pCU1 (Augustin), carrying cap5E; external plasmid standard for real time PCR

This work

pCapDvorne

pEPSA5 (Forsythe) harbouring a cap5D antisense fragment

This work

pCapAre

pCU1 harbouring cap5A

[34]

pCap5E

pCU1 harbouring cap5E

[34]

pCG132

pMUTIN4 (Vagner 1998) habouring cap5A

This work

  1. aAbbreviations: CP, capsular polysaccharides; CHL, chloramphenicol; MET, methicillin; VAN, vancomycin; r, resistant; s, susceptible.