Strain/plasmid | Relevant genotype or phenotypea | Source/reference |
---|---|---|
S. aureus strains | ||
SA137/93A | Clinical hVISA isolate; METr, Northern German epidemic MRSA, MLST sequence type ST247 | [4] |
SA137/93G | Mutant VISA of SA137/93A; ΔSCCmec (METs), ST247 | |
SA137/93G pCapDvorne | SA137/93G carrying pCapDvorne (CHLr) | This work |
Mu50 | Clinical VISA isolate | [29] |
SA1450/94 | Northern German epidemic MRSA, Iberian clone, ST247, CP5 positive, VANs control | German reference centre for staphylococci, Wernigerode |
SA1450/94 pCapAre | SA1450/94, repair of cap5A | This work |
SA1450/94 pCU1 | SA1450/94, control (empty vector) | This work |
Newman | CP5 positive, VANs | NCTC8178 |
Newman-132 | pMUTIN integrated into capsule promoter | This work |
NCTC8325 (RN1) | laboratory strain | National collection of type cultures |
HG001 | NCTC8325, repaired in rsbU | [31] |
HG001 pCap5E | HG001 capsule production repaired | This work |
SG511-Berlin | sensitive antibiotic screening strain | [32] |
RN4220 | Restriction negative derivative of NCTC8325, cloning host | [33] |
Plasmids | ||
pUC19gyrB | pUC19, carrying a 560 bp internal fragment of gyrB; external plasmid standard for real time PCR | This work |
pCU1cap5E | pCU1 (Augustin), carrying cap5E; external plasmid standard for real time PCR | This work |
pCapDvorne | pEPSA5 (Forsythe) harbouring a cap5D antisense fragment | This work |
pCapAre | pCU1 harbouring cap5A | [34] |
pCap5E | pCU1 harbouring cap5E | [34] |
pCG132 | pMUTIN4 (Vagner 1998) habouring cap5A | This work |