Figure 2
Transcript quantification of the essential capsule biosynthesis gene cap5E by real time PCR. a) Transcript amounts of cap5E throughout the growth curve of hVISA SA137/93A (filled square), VISA SA137/93G (filled triangle), VSSA Newman (filled circle) and VSSA SA1450/94 (filled diamond) indicated as copy number per 106 copies of the housekeeping gene gyrB. b) Transcript amounts of cap5E of VSSA strains (R: Reynolds*, N: Newman, 14: SA1450/94) and VISA strains (A: SA137/93A*, G: SA137/93G*, Mu: Mu50) at OD600 = 1 and OD600 = 4–5 indicated as copy number per 106 copies of gyrB. * Error bars are not visible at OD600 = 1 because of minimal data variations.