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Table 1 Oligonucleotide primers used in this study

From: Enrichment and characterization of a bacterial culture that can degrade 4-aminopyridine

Primer

Sequence (5' to 3')

Reference

pA

AGAGTTTGATCCTGGCTCAG

[7]

(8–28)

pH’

AAGGAGGTGATCCAGCCGCA

[7]

(1542–1522)

PRBA338GCf

CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGACTCCTACGGGAGGCAGCAG

This study

PRBA338f

TACGGGAGGCAGCAG

[26]

PRUN518r

ATTACCGCGGCTGCTGG

[26]

PRSTY1 a

ACGATAATGACGGTACCCGG

This study

PRSTY2 a

TTAGCCGGGACTTATTCTCC

This study

PRSTZ1 b

TACTTACGTGTAAGTAGCTGAAGG

This study

PRSTZ2 b

CCTTCAGCTACTTACACGTAAGTA

This study

PydAf c

GAYGAYCAYTTYGARAAYCA

This study

PydAr c

CATICCRCADATCCAYTC

This study

  1. a Used for amplification of the full-length 16S rRNA gene from strain 4AP-Y.
  2. b Used for amplification of the full-length 16S rRNA gene from strain 4AP-Z.
  3. c PydAf and PydAr were designed based on the conserved regions of 3-hydroxy-4-pyridone dioxygenase (3,4-dihydroxypyridine 2,3-dioxygenase), DDHFENH and EWICGM, respectively. R is A or G; Y is C or T; D is A, G, or T; and I is inosine.
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BMC Microbiology

ISSN: 1471-2180

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