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Table 2 Amino acid utilization from peptides (Trypticase) and amino acids by mixed human faecal bacteria in vitro with and without added 5 μM monensin

From: Ammonia production by human faecal bacteria, and the enumeration, isolation and characterization of bacteria capable of growth on peptides and amino acids

 

Amino acids

Amino acids + monensin

Trypticase

Trypticase + monensin

 

P value

 
 

Meana

SE

Mean

SE

Mean

SE

Mean

SE

Trypticase vsamino acids

Effect of monensin, amino acids

Effect of monensin, trypticase

ASP

0.673

0.171

0.650

0.170

0.754

0.159

0.570

0.160

NS

NS

0.050

GLU

1.460

0.367

−0.155

0.153

1.356

0.363

0.532

0.276

NS

0.005

0.006

SER

0.804

0.103

0.539

0.148

0.735

0.106

0.535

0.130

NS

NS

NS

GLY

0.414

0.086

0.056

0.044

0.386

0.052

0.092

0.039

NS

0.005

0.001

HIS

0.178

0.030

0.055

0.023

0.200

0.029

0.077

0.029

NS

0.006

0.018

ARG

0.255

0.034

0.217

0.042

0.347

0.035

0.339

0.070

NS

NS

NS

THR

0.361

0.083

0.156

0.047

0.626

0.063

0.343

0.080

0.005

0.023

0.007

ALA

0.139

0.053

−0.027

0.041

0.207

0.042

0.032

0.050

NS

0.034

0.000

PRO

0.468

0.157

0.067

0.100

0.685

0.171

0.055

0.094

NS

0.013

0.012

TYR

0.078

0.031

0.024

0.019

0.062

0.013

0.031

0.014

NS

0.015

0.009

VAL

0.132

0.062

0.026

0.051

0.153

0.037

0.070

0.042

NS

NS

NS

ILE

0.140

0.054

0.040

0.040

0.178

0.038

0.088

0.023

NS

0.022

NS

LEU

0.278

0.097

0.151

0.098

0.343

0.082

0.250

0.097

NS

0.025

NS

PHE

0.094

0.031

0.042

0.024

0.149

0.031

0.082

0.015

NS

0.014

NS

LYS

0.542

0.130

0.396

0.146

0.764

0.166

0.498

0.164

0.043

0.014

NS

Total

6.017

1.214

2.237

0.907

6.946

0.976

3.596

0.658

NS

0.011

0.005

  1. aμmol amino acid metabolised h-1 ml-1, n = 6.
  2. NS, P > 0.05.