A high throughput multiplex PCR assay for simultaneous detection of seven aminoglycoside-resistance genes in Enterobacteriaceae

Table 3 Primers for the entire coding regions of 7 aminoglycoside-resistance genes

Primer	Sequence (5'→3')	Reference or source	Size (bp)
aac(3)-II	F: ATATCGCGATGCATACGCGG	[31]	877
aac(3)-II	R: GACGGCCTCTAACCGGAAGG	[31]	877
aac(6’)-Ib	F: TTGCGATGCTCTATGAGTGGCTA	[32]	472
aac(6’)-Ib	R: CTCGAATGCCTGGCGTGTTT	[32]	472
aac(6’)-II	F: CGACCATTTCATGTCC	This study*	542
aac(6’)-II	R: GAAGGCTTGTCGTGTTT	This study*	542
ant(3″)-I	F: CATCATGAGGGAAGCGGTG	[33]	787
ant(3″)-I	R: GACTACCTTGGTGATCTCG	[33]	787
aph(3’)-VI	F: ATGGAATTGCCCAATATTATT	[34]	780
aph(3’)-VI	R: TCAATTCAATTCATCAAGTTT	[34]	780
armA	F: CCGAAATGACAGTTCCTATC	[13]	846
armA	R: GAAAATGAGTGCCTTGGAGG	[13]	846
rmtB	F: ATGAACATCAACGATGCCCTC	[13]	769
rmtB	R: CCTTCTGATTGGCTTATCCA	[13]	769

*The primers have been validated with referenced strains (GU944731.1 and HQ880255.1).

ISSN: 1471-2180