Figure 2

The specificity of GeXP assay for the detection of aac(3)-II, aac(6′)-Ib, aac(6′)-II, ant(3″)-I, aph(3′)-VI, armA and rmtB. Seven recombinant plasmids harboring aminoglycoside-resistance genes were respectively detected via the GeXP assay. All the specific peaks were observed presenting the gene-specific target amplicons of aac(3)-II, aac(6′)-Ib, aac(6′)-II, ant(3″)-I, aph(3′)-VI, armA and rmtB, respectively (a~g). The negative control assay clearly showed the DNA size standard from 140 to 420 nt (peaks in red color) without nonspecific products presented (h).