Figure 2

CPP-mediated GFP delivery in cyanobacteria. (a) The 6803 and 7942 strains of cyanobacteria were treated with GFP only or R9/GFP mixtures for 20 min at room temperature. (b) GFP delivery in the presence of the endocytic inhibitor NEM. Cells were pretreated with NEM, and then either GFP only or R9/GFP was added to cells for 20 min. Green and red fluorescence were detected in GFP and RFP channels using a Leica confocal microscope at a magnification of 1,000× (a and b). (c) Histogram of relative fluorescent intensity. Green fluorescence detected in the cells treated with only GFP served as a control. Fluorescent intensity detected in experimental groups was compared to that of the control group. Data are presented as mean ± SD from three independent experiments. Significant differences were set at P < 0.05 (*) or 0.01 (**).