Skip to main content
Figure 2 | BMC Microbiology

Figure 2

From: Integrin α5β1-fimbriae binding and actin rearrangement are essential for Porphyromonas gingivalis invasion of osteoblasts and subsequent activation of the JNK pathway

Figure 2

Actin filament rearrangement is essential for P. gingivalis invasion of osteoblasts. A. Osteoblast nuclei, actin and P. gingivalis are indicated by blue, red or green fluorescence, respectively. No appreciable change in actin filament organization was seen 30 min after infection. At 3 h, actin relocated to the periphery of the osteoblasts, leaving a void space surrounding the osteoblast nuclei occupied by P. gingivalis. Twenty-four hours after infection, actin became more condensed and formed a cortical outer shell. The number of perinuclear P. gingivalis was also significantly increased. Addition of the actin disrupting agent, cytochalasin D, reduced the number of osteoblasts with P. gingivalis invasion. Notice that actin had now become disorganized, as demonstrated by the punctuated pattern. B. Quantitative analysis of confocal images demonstrated that P. gingivalis invasion of osteoblasts was inhibited by the disruption of actin filaments. Abbreviations: min, minute; h, hour; Ctrl and CT, control, non-infected osteoblasts; PG, P. gingivalis. Scale bar = 20 μm. * denotes P < 0.05.

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us