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Table 1 Bioengineered L. jensenii derivatives with the expression cassette stably integrated into the bacterial chromosome

From: Homeostatic properties of Lactobacillus jensenii engineered as a live vaginal anti-HIV microbicide

Strain

Integration Site

Expression Cassette

  

Promoter

Integrated gene

L. jensenii 1153a

NAb

NA

NA

L. jensenii 1153-1666

pox1

rpsU

APVT-CV-N (P51G)

L. jensenii 1153-2666

pox1

ptsH

APVT-CV-N (P51G)

L. jensenii 1153-3666

pepO

rpsu

APVT-CV-N (P51G)

L. jensenii 1153-1646

pox1

gusA

Gus A (β-glucoronidase)

L. jensenii 1153-GFP

pox1

rpsU

EGFPc

  1. aParental L. jensenii strain; bNA=not applicable (wild type strain); cenhanced green fluorescent protein.