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Table 3 Oligo nucleotides used for mutagenesis

From: Release of extracellular ATP by bacteria during growth

Oligonucleotides

Used for

Sequence

cyoA5KO

Mutagenesis of cyoA

5′-ctcaggaaatacaataaaagtttgggatggttgtcattaattgcaggcactgcattactcagtggct gtaattctgcgctgctggatcccgtgtaggctggagctgcttc-3′

cyoA3KO

Mutagenesis of cyoA

5′-caacccttggagttggcggattccgcgtggctcatgtccataccttccattccttcatgcgagctgtgc tcaccttcaggttgggtcatgcatatgaatatcctccttag-3′

cyoB5KO

Mutagenesis of cyoB

5′-ataataagcaatcgttgcctgcgattaccctcgcagctattggggttgtctacggtgatattggt accagcccgctttatacgcttcgtgaatgtttgtcgtgtaggctggagctgcttc-3′

cyoB3KO

Mutagenesis of cyoB

5′-aaattatcactggatgcagtaccgttccatgaacctatcgtcatggtaacgatcgctgcaattatcgtc gggggactggcgatactggcagtgtaggctggagctgcttc -3′

cyoC5KO

Mutagenesis of cyoC/D mutation

5′-tgcattctgttctctattctgtttgctacctatgccgttctggtgaacggcaccgctggcggcccgacaggt aaggacattttcgaactggtgtaggctggagctgcttc -3′

cyoD3KO

Mutagenesis of cyoC/D mutation

5′-tgtagttgaggttccacataatccagatggagcccacaaccaggatggcgatgatcagcacggtaaag atgaaggccgtcatgttccagcatatgaatatcctccttag -3′

cyoA5

Confirmation of cyoA mutation

5′-atcatgtttacagtaatgta-3′

cyoA3

Confirmation of cyoA mutation

5′-tccgaacatcttatcttcct -3′

cyoB5

Confirmation of cyoB mutation

5′-aggaagataagatgttcgga-3′

cyoB3

Confirmation of cyoB mutation

5′-tcgcgtgcgttaaagtatca -3′

cyoCD5

Confirmation of cyoC/D mutation

5′-tgatactttaacgcacgcga -3′

cyoCD3

Confirmation of cyoC/D mutation

5′-tgcaggtattgcttaaacat-3′

K1

KanR primer for confirmation of mutation

5′-cagtcatagccgaatagcct-3′

K2

KanR primer for confirmation of mutation

5′-cggtgccctgaatgaactgca-3′