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Figure 5 | BMC Microbiology

Figure 5

From: Release of extracellular ATP by bacteria during growth

Figure 5

Live bacteria deplete ATP in the culture medium. (A) and (B) Live or heat – killed E. coli K12 (A) or Salmonella SE2472 (B) were spun down and incubated at 37°C in fresh LB supplemented with 10 μM ATP. Culture supernatant from live bacteria was supplemented with ATP to 10 μM. ATP depletion by bacteria cells or culture supernatant was measured by the residual ATP level in culture medium after various culture periods of incubation at 37°C. The residual ATP levels were plotted against the incubation period. (C) and (D) Free and cell-associated ATP in E. coli (C) or Salmonella (D) culture incubated with S35-α-ATP or P32-γ-ATP. The relative levels of radioactivity in culture supernatant and bacterial cells were determined and plotted against the incubation period. Each experiment was performed three times and results are from a representative experiment.

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