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Figure 4 | BMC Microbiology

Figure 4

From: Release of extracellular ATP by bacteria during growth

Figure 4

The ∆cyo mutants of E. coli BW25113 and Salmonella SE2472 have lower extracellular ATP levels during growth. Overnight cultures of wild type (WT) or ∆cyo mutants of E. coli (A and B) or Salmonella (C and D) were diluted 1:100 in fresh LB broth and cultured at 37°C with shaking. Aliquots were collected at various time points and ATP assays were carried out with culture supernatant or whole culture. The ATP levels in the culture supernatant (A and C) or whole culture (B and D) were normalized using OD600nm and plotted against the incubation period. Results are the average of 3 experiments and error bars represent standard deviations.

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