Figure 1From: Release of extracellular ATP by bacteria during growthATP is present in the bacterial culture supernatant and the extracellular ATP is not due to bacteria contamination. Overnight culture of Salmonella strain SE2472 was diluted 1:100 in LB and cultured at 37°C for 3 hours with shaking to reach early log phase. The overnight (stationary) and 3 hour (early log phase) cultures were spun down. An aliquot of each culture supernatant was filtered through a 0.22 μm filter to remove any residual bacteria. ATP levels in the filtered (hatched bars) or unfiltered culture supernatant (open bars) were measured. Results are the average of 3 assays and error bars represent standard deviations.Back to article page