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Table 2 Range and levels of independent variables lysine (Lys), 1,3-diaminopropane (1,3D), cadaverine (Cad), and putrescine (Put), in coded and original units, according to two-factor, three-level central-composite-based, face-centered, experimental designs (CCF); the response variable is cephamycin C concentration (CephC) obtained at 72-hour cultivation

From: Enhancing effect of lysine combined with other compounds on cephamycin C production in Streptomyces clavuligerus

 

Independent variables

Response

 

Coded units

Original units (g l-1)

CephC (mg l-1)

 

Lys + 1,3D

Lys + Cad

Lys + Put

Run

x Lys

x i

x Lys

x 1,3D

x Cad

x Put

Measured*

Predicted

Measured*

Predicted

Measured*

Predicted

1

-1

-1

0.0

0.0

0.0

0.0

18.1 ± 3.0

10.6

19.0 ± 2.7

22.7

18.0 ± 2.7

16.7

2

0

-1

3.7

0.0

0.0

0.0

45.6 ± 7.2

59.9

45.6 ± 2.2

39.1

47.3 ± 3.2

53.9

3

+1

-1

7.4

0.0

0.0

0.0

72.3 ± 4.1

64.9

72.1 ± 1.9

74.7

75.5 ± 3.6

70.3

4

-1

0

0

2.5

3.5

0.2

47.6 ± 3.9

53.9

34.7 ± 3.5

30.2

31.1 ± 2.2

33.8

5

0

0

3.7

2.5

3.5

0.2

108.9 ± 0.0

109.2

40.5 ± 0.0

41.2

63.1 ± 0.0

64.6

6

0

0

3.7

2.5

3.5

0.2

122.1 ± 0.0

109.2

35.9 ± 0.0

41.2

75.0 ± 0.0

64.6

7

0

0

3.7

2.5

3.5

0.2

100.7 ± 0.0

109.2

42.0 ± 0.0

41.2

69.0 ± 0.0

64.6

8

0

0

3.7

2.5

3.5

0.2

120.0 ± 0.0

109.2

41.1 ± 0.0

41.2

64.9 ± 0.0

64.6

9

+1

0

7.4

2.5

3.5

0.2

114.4 ± 13.6

120.2

74.2 ± 2.1

71.5

64.0 ± 3.4

74.7

10

-1

+1

0

5.0

7.0

0.4

62.2 ± 2.6

62.4

47.1 ± 2.8

47.9

29.1 ± 2.5

27.5

11

0

+1

3.7

5.0

7.0

0.4

125.3 ± 0.8

123.7

54.1 ± 0.2

53.5

44.9 ± 2.9

51.9

12

+1

+1

7.4

5.0

7.0

0.4

140.2 ± 8.0

140.7

78.8 ± 0.5

78.5

61.1 ± 1.9

55.6

  1. *The cultivations were performed in triplicate, with the exception of cultivation at condition (0,0) performed in quadruplicate; SD = standard deviation.